Flp-intm t-rextm 293 细胞系

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August undefined, 2024

WebFlp-InTM T-RExTM 293 cells stably harboring hFFA2-eYFP at the Flp-InTM T-RExTM locus were induced to express the receptor. The capacity of GLPG0974 to inhibit elevation of [Ca2+] i. produced by EC80 concentrations of C3 or Cmp 1 is shown (B). Membranes from such induced cells were used to assess the ability of GLPG0974 to inhibit the binding of ... WebBioz Stars score, Techniques, Protocol Conditions and more for Flp Intmt Rextm 293 Cells Hek 293t Hek Human Embryonic Kidney 293 Cells, supplied by Thermo Fisher. Data for …

FlpIn™系统FlpI_价格-品牌-详情介绍_丁香通

Web技术资料. 规格：. 1ml. Flp-In™系统使您可以稳定整合和表达感兴趣的基因，以提供单拷贝等基因细胞系。. Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶 … how to stay slim forever

Flp-In™ 系统 Thermo Fisher Scientific - CN

WebHuman. The Flp-In T-REx-293 cell line stably expresses the lacZ-Zeocin fusion gene and the Tet repressor, and is designed for use with the Flp-In T-REx System. The cell line expresses the Tet repressor from pcDNA6/TR and contains a single integrated Flp Recombination Target (FRT) site from pFRT/lacZeo as confirmed by Southern blot analysis. WebSep 29, 2024 · 293FTM 细胞是由 Flp-InTM T-RExTM 293 ... 总之， 293系列细胞经过长时间的改造与优化，已在多个领域实现了较多的应用，源井生物对于293与293T细胞也有丰富的基因编辑和细胞模型构建的经验，并拥有上千种293细胞的KO现货，覆盖超20种信号通 … http://www.bio-thing.com/p118025 react render function pattern

HEK293系列细胞的前世今生，源井给你293细胞家族最全盘点！

WebThe Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened by hygromycin B resistance. Western blot and indirect immunofluorescence (IFA) were used to examine the expression of the target protein in ... WebFeb 20, 2024 · 在我们以前的工作中，我们在四环素诱导型CMV启动子（HEK-293 Flp-In T-REx-Thermo）的控制下，建立了表达C末端3xFLAG标记的外来体成 … how to stay slim without dietingWeb293FTM细胞则是来源于Flp-InTMT-RETM293细胞，该细胞系中转染了ecotropic receptor质粒及MAPPIT(mammalian protein-protein interaction trap)报告质粒，该细胞系主要用于 … react render function output

"WebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows … " - Flp-intm t-rextm 293 细胞系

Flp-intm t-rextm 293 细胞系

哺乳动物表达基础支持 - 入门 Thermo Fisher Scientific - CN

WebOct 31, 2024 · HEK293细胞及其衍生株HEK293（human embryonic kidney 293），简称293细胞，来源于人胚胎肾细胞，由于在19号染色体整合了大约4.35 kb的腺病毒基因组片段，使其细胞周期以及细胞表型、核型等发生了 ... 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性（ecotropic）报告质粒和 ... WebFlp-In™系统使您可以稳定整合和表达感兴趣的基因，以提供单拷贝等基因细胞系。Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶标（FRT）位点。随后，包含您感兴趣的基因的表达载体会通过FRT位点的Flp重组酶介导DNA重组，整合入基因组。

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WebT-REx™ 细胞系可稳定表达四环素抑制子蛋白（表 1）。使用 T-REx™ 系统可以节省大量的时间和精力。通过使用阳性对照载体 pcDNA™4⁄TO⁄lacZ 进行瞬时转染，对 T-REx™ 细胞系进行功能检测。在用四环素诱导时，T-REx™ 细胞系在抑制状态下表现出极低的基础表达水平，表达水平较高（图 1）。 WebAug 21, 2024 · 最近，有研究通过RMDI技术在Flp-InTM-293细胞中系成功地过表达核孔复合物亚基，以及在Flp-InTM-CHO细胞中表达P2Y12受体。已有研究构建了具有两个不同重组位点（双重RMCE）的宿主细胞，用于在单个细胞系中过表达多个外源基因。

WebFigure 4-2. TUG-891 but not TUG-1197 displays low potency mediated activation of hFFA1. Flp-InTM T-RExTM 293 cells able to express hFFA1-mVenus-HA were seeded (75,000 cells /well) into poly-D-lysine coated black clear-bottom 96-well plates. Expression of hFFA1 was induced by the antibiotic doxycycline (100 ng/ml) for 18-24 hours. Cells were … WebAbstract. Each subtype of the muscarinic receptor family of G protein-coupled receptors is activated by similar concentrations of the neurotransmitter acetylcholine or closely rel

Webgenomic location. To generate these cell lines, the Flp-In ™ T-REx System involves the following major steps: 1. Independent integration of the following two plasmids into the … WebThe Flp-In ™ T‑REx -293 cell line is shipped on dry ice. Store in liquid nitrogen vapor-phase upon receipt. The Flp-In™ T‑REx™-293 cell line is supplied as one vial containing 1 × …

WebSep 29, 2024 · 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性（ecotropic）报告质粒和MAPPIT (mammalian protein-protein interaction trap) 报告质粒获得，常用于蛋白互作筛选。 293S 细胞. 293S细胞支持悬浮培养，S为Suspension悬浮的首字母，可以耐受低Ca2⁺培养体系。

WebFeb 13, 2024 · flp/frt 系统就属于位点特异性重组系统的一种。 FLP是一个重组酶（flippase，Flp），类似于Cre，它识别的位点是FRT，当FRT在同一条DNA上且方向相 … how to stay smallWebOct 25, 2024 · The Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened ... react render html fileWebFeb 20, 2024 · 在我们以前的工作中，我们在四环素诱导型CMV启动子（HEK-293 Flp-In T-REx-Thermo）的控制下，建立了表达C末端3xFLAG标记的外来体成分EXOSC10（RRP6）的同基因HEK-293细胞系费雪科学）。. 该系统允许我们以与内源WT蛋白质相当的水平表达标记的EXOSC10蛋白质，并且使用磁性 ... react render function with parametershttp://www.bio-thing.com/p118025 how to stay small in heightWebApr 2, 2024 · 基本信息. 商品名称：Epigenetics in Cancer 作者：编者:JUNJIANG FU//SABER IMANI 定价：198 出版社：科学 ISBN号：9787030543769 其他参考信息（以实物为准）. 出版时间：2024-01-01 印刷时间：2024-01-01 版次：1 印次：1 开本：16开包装：平装页数：364 内容提要 react render functional componentWeb丁香通为您提供FLPINTREX293商品详情介绍：价格：￥1 - 2000，货号：R78007，品牌：invitrogen,GIBCO，产地：美国，详见丁香通FLPINTREX293商品详情页； react render html from apiWebFlp-In ™ 293 T-REx 细胞系设计用于快速生成稳定细胞系，确保通过 Flp-In™ 表达载体对您的目标蛋白质进行均一表达。这些细胞在转录活性基因组基因座处包含一个稳定整合的 … react render html from string